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At this point it cannot be ruled out that some of the proteins that were captured with monothiol glutaredoxin mutants as Grx(SSR) species might have just accumulated as unspecific dead-end products
After being boosted through precursor supplementation, NAD+ often plateaus and declines around week 12, which is likely due to an overabundance of NAM (a byproduct of NAD+) and a shortage of methyl donors present to fuel conversion in the salvage pathway.38 Supplementing with a potent methyl donor can offset this shortage and ensure sustained activity in the salvage pathway
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