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The present study observed reduced expression of 25-hydroxylase enzymes and 1--hydroxylase, vitamin D receptor, and increased expression of the 24-hydroxylase enzyme in the livers of HFD-fed mice
These provide objective data on fat mass versus lean mass changes that the scale alone cannot reveal
Contrast that with a patient eating a varied omnivorous diet with no B12 risk factors who receives the same formulation
At the end of all incubations, the hECs were washed three times with ice-cold PBS containing 0.1% BSA, once with acid stripping buffer (glycine 50 mM, NaCl 100 mM, pH 3) to remove the non-internalized, but cell surface-attached nanocarriers, and finally with PBS
Some of the dissected cerebral cortex and striatum were quickly immersion-perfused with 4% paraformaldehyde in 0.1 M phosphate-buffered saline (PBS) for 2 days and then transferred to 15% sucrose solution in PBS containing 0.1% sodium azide at 4 C
