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qPCR was performed with SYBR Green Supermix (TaKaRa) with the protocol as follow: 95 C for 5 min, repeating 40 cycles of 95 C for 5 s and 60 C for 45 s using StepOnePlus real-time PCR system (Applied Biosystem, Foster City, CA, USA)
The prevalence is similar globally, he explained, although exact figures can vary based on regional health demographics and reporting practices. Most commonly, it affects middle-aged or older white people
doi: 10.1111/dom.15585 171 TaneeraJJinZJinYMuhammedSJZhangELangSet al
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