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After precipitating the serum, the serum was centrifuged at 12 000 r/min for 10 min, and then the serum was taken and stored at -80C for spare

Thiocyanate is a degradation product of chemicals found in cigarette smoke, in vegetables (such as cassava), and, generally, in different types of food

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Research Handling Note: Allow peptide and solvent to reach room temperature before reconstitution to maintain structural integrity

These additional compounds each require independent calculation, expanding the planning needed but not changing the fundamental math for any single peptide