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Consistent with this finding, no improvement in glucose tolerance was observed during an intraperitoneal glucose tolerance test in C57BL6 mice that were administered a subcutaneous dose of PF-06882961 (10 mg/kg) (Fig

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After treated with test compounds for 15 hours, harvested in 5 ml Dulbecco s modified medium containing Deep Red Reagent (5 M) (Molecular Probes, Invitrogen) and incubated for 30 min at 37C in a tissue culture incubator