Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
Additionally, a pooled analysis of large-scale clinical studies evaluating the cardiorenal advantages of GLP-1RAs in T2DM suggested a trend towards reduced pulmonary adverse events, including asthma, in the GLP-1RAs treatment groups [256]
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Nevertheless, other organs/fluids like muscle, gut/intestine, plasma or mucus have also been successfully used in comparative proteomics studies (de Vareilles et al., 2012a
Protective effects of ginkgo biloba against acetaminophen-induced toxicity in mice.Mol Cell Biochem