All slides were examined by a blinded histopathologist under standardized conditions (400 magnification, identical illumination and calibration), and ten random microscopic fields per sample were evaluated to ensure objectivity and reproducibility
: : 81 3 (85 ) : 24 3 (85 ) : 5 3 (85 ) : 3 3 (85 ) : 8 8 (227 )
The model was first fitted to the training concentrations (37.5 and 25 M doxorubicin), and then used to simulate the test concentrations (50, 12.5 and 10 M doxorubicin) as a means of blind validation, showing good accordance between the experimental and in silico outputs for all concentrations of doxorubicin
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