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Without knowing where you started, it becomes difficult to objectively assess whether your protocol is working as intended

Automated single-particle data acquisition was performed with the EPU (Thermo Fisher Scientific) software at a nominal magnification of 105,000 , yielding a final pixel size of 0.83

Lyophilized (freeze-dried) peptide should be stored at minus 20 degrees Celsius (minus 4 degrees Fahrenheit) for long-term storage

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