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In this regard, we speculate that the irrelevance of this study may stem from the method of Se measurement, which is available in whole blood, serum (79, 80), urine (81), nails (82), and questionnaires (83)
Two habits prevent most misreadings
A standard curve was created using serial dilutions of plasmid DNA containing the target mtDNA sequence, and amplification efficiency was validated with a correlation coefficient above 0.98
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