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Glutathione is a naturally occurring tripeptide made from glutamate, cysteine, and glycine

Richie: GSH is technically a peptide, which is like a very small protein, and it is made up of three amino acids (cysteine, glutamic acid and glycine) that are linked together

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Spiking experiments involved addition of known amounts of pure NAD and glutathione compounds individually at different stages of the extraction (Supplementary Table 1), followed by quantification using colorimetric assays

Decide whether your current concentration delivers satisfactory progress or whether increasing concentration might accelerate results