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Add each solvent one by one: 10% DMSO 90% (20% SBE--CD in Saline) Solubility: 2.5 mg/mL (9.34 mM)

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The column eluate was nanosprayed into a Sciex 5600 + Triple-TOF mass spectrometer with gas settings of Ion source gas1 set at 3, gas2 set at zero, and Curtain Gas set at 25

Lixisenatide comprises 44 amino acids and is based on the Ex-4 peptide sequence, omitting proline at position 36 and adding six lysine residues at the C-terminal (110)